DNR officers also have access to the Department of Public Safety talkgroups and will usually use those for dispatching purposes.  These DNR talkgroups are mainly for unit to unit coordination.

District 1 contains Buena Vista, Calhoun, Cherokee, Clay, Dickinson, Emmet, Hancock, Humboldt, Ida, Kossuth, Lyon, O'Brien, Osceola, Palo Alto, Plymouth, Pocahontas, Sac, Sioux, Webster, Winnebago, Woodbury, Worth, and Wright counties.

District 2 contains Allamakee, Black Hawk, Bremer, Buchanan, Butler, Cerro Gordo, Chickasaw, Clayton, Delaware, Dubuque, Fayette, Floyd, Franklin, Howard, Jackson, Jones, Mitchell, and Winneshiek counties.

District 3 contains Adair, Adams, Audubon, Carroll, Cass, Clarke, Crawford, Dallas, Decatur, Fremont, Greene, Guthrie, Harrison, Madison, Mills, Montgomery, Monona, Page, Pottawattamie, Ringgold, Shelby, Taylor, and Union counties.

District 4 contains Benton, Cedar, Clinton, Des Moines, Henry, Iowa, Jefferson, Johnson, Keokuk, Linn, Lee, Louisa, Muscatine, Scott, Tama, Van Buren, and Washington counties.

District 5 contains Appanoose, Boone, Davis, Grundy, Hamilton, Hardin, Jasper, Lucas, Mahaska, Marion, Marshall, Monroe, Polk, Poweshiek, Story, Wapello, Warren, and Wayne counties.