| BNSF Railway | Burlington Northern Santa Fe | 41.97991 | -93.55408 | 200 |
| Canadian National Railway | General Operations | 42.56117 | -93.70239 | 165 |
| Norfolk Southern | Norfolk Southern | 41.60054 | -93.60911 | 10 |
| Union Pacific | Blair Subdivision | 41.54876 | -95.9692 | 7 |
| BNSF Railway | BNSF Council Bluffs Yard | 41.26194 | -95.86112 | 2.5 |
| BNSF Railway | BNSF Fort Madison Yard | 40.6208 | -91.34612 | 2.5 |
| BNSF Railway | BNSF West Quincy Yard | 39.91916 | -91.44077 | 1 |
| Union Pacific | Boone Subdivision | 41.74746 | -94.74214 | 75 |
| Union Pacific | Clinton Subdivision | 41.93534 | -91.51963 | 75 |
| Canadian Pacific Railway | Davenport Subdivsion | 41.48753 | -90.63792 | 25 |
| Union Pacific | Estherville Subdivision | 43.03896 | -94.41826 | 35 |
| Union Pacific | Fairmont Subdivision | 41.42757 | -92.9584 | 200 |
| Union Pacific | Falls City Subdivision | 41.42757 | -92.9584 | 200 |
| Union Pacific | Fort Dodge Subdivision | 41.42757 | -92.9584 | 200 |
| Union Pacific | Jewell Subdivision | 41.42757 | -92.9584 | 200 |
| Canadian Pacific Railway | Laredo Subdivsion | 40.58968 | -93.37983 | 75 |
| Union Pacific | Laurens Subdivision | 41.42757 | -92.9584 | 200 |
| Canadian Pacific Railway | Marquette Subdivsion | 42.07113 | -90.17402 | 25 |
| Union Pacific | Mason City Subdivision | 41.42757 | -92.9584 | 200 |
| Canadian Pacific Railway | Mason City Subdivsion | 43.06158 | -92.22627 | 50 |
| Union Pacific | Omaha Subdivision | 41.42757 | -92.9584 | 200 |
| Union Pacific | Oskaloosa Subdivision | 41.42757 | -92.9584 | 200 |
| Canadian Pacific Railway | Ottumwa Subdivsion | 41.23275 | -91.41526 | 55 |
| Canadian Pacific Railway | Owatonna Subdivsion | 43.68809 | -93.20054 | 40 |
| Union Pacific | Perry Subdivision | 41.42757 | -92.9584 | 200 |
| Union Pacific | Rake Subdivision | 41.42757 | -92.9584 | 200 |
| Canadian Pacific Railway | Sheldon Subdivsion | 43.14144 | -94.30466 | 60 |
| Union Pacific | Sioux City Subdivision | 41.42757 | -92.9584 | 200 |
| Union Pacific | Tara Subdivision | 41.42757 | -92.9584 | 200 |
| Union Pacific | Trenton Subdivision | 41.42757 | -92.9584 | 200 |
| Canadian National Railway | Waterloo Terminal | 42.49244 | -92.34616 | 5 |
| Union Pacific | Worthington Subdivision | 41.42757 | -92.9584 | 200 |
| Canadian Pacific Railway | Eldridge Spur | 41.65809 | -90.58458 | 5 |
| Iowa Interstate Railroad | Cedar Rapids Subdivision | 41.82492 | -91.75583 | 20 |
| Iowa Interstate Railroad | Council Bluffs Subdivision | 41.4431 | -94.78257 | 60 |
| Iowa Interstate Railroad | Iowa City Subdivision | 41.6076 | -91.36033 | 45 |
| Iowa Interstate Railroad | Newton Subdivsion | 41.67738 | -92.59629 | 52 |
| Iowa River Railroad | Operations | 42.47721 | -93.05533 | 6 |
| Burlington Junction Railway | Operations | 41.00892 | -91.75781 | 45 |
| Iowa Southern Railway | Operations | 40.75057 | -92.86687 | 30 |
| Cedar Rapids and Iowa City Railway | Operations | 41.79998 | -91.62598 | 20 |
| Iowa Northern Railway | Operations | 42.71473 | -92.30713 | 75 |
| Iowa Traction Railway | Operations | 43.14417 | -93.28716 | 5 |
| Waterloo Railroad | Operations | 42.59464 | -92.10458 | 15 |
| Boone and Scenic Valley Railroad | Operations | 42.09975 | -93.92349 | 7 |
| Midwest Central Railroad | Operations | 40.96364 | -91.55794 | 2 |
