| Appalachian and Ohio | Road Operations | 38.79399 | -80.53203 | 25 |
| Appalachian and Ohio | Buckhannon Yard | 38.99399 | -80.23203 | 5 |
| CSX Transportation | Systemwide | 38.93036 | -80.41772 | 147.7 |
| CSX Transportation | Baltimore Division - Road | 39.34092 | -80.01897 | 100 |
| CSX Transportation | Baltimore Division - Grafton Terminal | 39.34092 | -80.01897 | 5 |
| CSX Transportation | Florence Division - Road | 38.34982 | -81.63262 | 75 |
| CSX Transportation | Florence Division - Huntington Terminal | 38.41925 | -82.44515 | 5 |
| Kanawha River Railroad | Road Operations | 38.21816 | -81.44289 | 75 |
| Norfolk Southern | Systemwide | 38.93 | -80.42 | 147.7 |
| Norfolk Southern | Pocahontas Division - Road | 37.67427 | -81.96736 | 60 |
| Norfolk Southern | Pocahontas Division - Bluefield Yard | 37.26984 | -81.22232 | 5 |
| Norfolk Southern | Pocahontas Division - Elmore Yard | 37.56678 | -81.40538 | 5 |
| Norfolk Southern | Pocahontas Division - Kenova Yard | 38.39897 | -82.57821 | 5 |
| Norfolk Southern | Pocahontas Division - Williamson Yard | 37.67427 | -82.27736 | 5 |
| Norfolk Southern | Harrisburg Division - Road | 39.37903 | -77.8974 | 25 |
| Shortline Railroads | Big Eagle Railroad | 38.10371 | -81.60262 | 10 |
| Shortline Railroads | Wheeling-Pittsburgh Steel Railroad | 40.06396 | -80.72091 | 5 |
| R.J. Corman West Virginia Lines | R. J. Corman Railroad | 37.90061 | -81.25888 | 25 |
